rabbit polyclonal antibody against p gp  (Danaher Inc)

Journal: PLoS ONE

Article Title: P-glycoprotein Modulates Morphine Uptake into the CNS: A Role for the Non-steroidal Anti-inflammatory Drug Diclofenac

doi: 10.1371/journal.pone.0088516

Figure Lengend Snippet: Animals received subcutaneous injections of saline (Sal) or λ-carrageenan (Carr) into the plantar surface of the right hind paw. 15 min after paw treatment animals received saline (Sal, 1 mL/kg, i.p.) or diclofenac (Diclo, 30 mg/kg, i.p.). 3 h after paw treatment animals were sacrificed and brain microvessels were isolated, plated on glass slides and fixed. Vessels were probed with mouse monoclonal anti-P-glycoprotein antibody and rabbit polyclonal antibody directed against von Willebrand factor (VWF). All slides were then incubated with appropriate Alexa Fluor-conjugated secondary antibodies. A . Representative immunofluorescent images from animals treated with saline paw and saline i.p. (Sal/Sal), saline paw and diclofenac i.p. (Sal/Diclo), λ-carrageenan paw and saline i.p. (Carr/Sal) or λ-carrageenan paw and diclofenac i.p. (Carr/Diclo). B . Semiquantitative analysis of the mean fluorescent intensity of P-gp. Microvessels isolated from treated and control animals were randomly selected for image analysis. Each bar represents the mean ± SEM of 75 microvessels from 3 separate experiments. Asterisks represent data points that are significantly different from Sal/Sal (*p<0.05).

Article Snippet: Rabbit polyclonal antibody against P-gp (H-241; 200 μg/mL), which recognizes amino acids 1040–1280 of P-gp derived from both mdr1a and mdr1b, was purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Saline, Isolation, Incubation, Control